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• Free Dna Sequence Analysis Software
• Dna Sequence Analysis Tools

CLC Main Workbench 5.6.1 provides you with a software environment which enables users to make a large number of advanced DNA, RNA, and protein sequence analysis, combined with gene expression analysis, smooth data management, and excellent graphical. Home » Products » Sequencher » Free Download To get your free 15-day evaluation license or to update your version of Sequencher to 5.4.6, follow these four easy steps. This will provide you with the full Sanger and NGS functionality for your DNA Sequencing.

Overview of Major Features

Protein sequences can be reverse translated into DNA, compared using 'Dot-Plot' analysis and scanned for Proteolytic cleavage sites and amino acid sequence motifs. A comprehensive Protein Analysis Toolbox provides a wide variety of algorithms for analyzing the composition of proteins and presenting the results in graphical and tabular formats. If you have any problems downloading or running DNADynamo email [email protected] About the free DNADynamo demo The free trial demo runs for 60 days, though extensions are available upon request. If you wish to keep using DNADynamo for DNA sequence analysis and sequencing after the evaluation has expired you need to purchase a license for the software.

If you already own an older copy of MacVector, and would like to know what new features have been added since the version you own, you can find that information on this summary page.

Graphical Sequence Editing

MacVector can read and write DNA and Protein sequences in most popular file formats. In addition to directly editing sequences and features/annotations, MacVector has an intuitive 'Click Cloning' graphical interface that lets you easily replicate laboratory cloning experiments to create new molecules. MacVector uses the native Mac OS X Quartz graphics to generate publication quality images that can be scaled to any size with no loss of resolution. More details..

Gateway and Topo Cloning

MacVector supports the popular Gateway, TOPO TA and Zero Blunt cloning technologies from Invitrogen. With a few simple clicks from an intuitive graphical interface, you can replicate your biological manipulations at the bench to create new molecules with the correct sequences across the cloning and recombination junctions. More details..

Auto Annotation

You can scan an unannotated or partially annotated sequence against a folder on your hard drive and MacVector will identify matching features in sequences in the target folder and add them to your sequence. Because MacVector includes custom feature appearance information when annotating the sequence, you can use this to maintain a carefully curated set of your favorite genes and sequences each with a graphical appearance that best suits your needs. More details..

Primer Design

You can design primers for either PCR or Sequencing/Hybridization probes using a variety of primer design functions, including an interactive manual design interface along with several scanning functions such as the popular Primer3 algorithm. The interactive interface clearly shows which potential primers may have secondary structure problems, alternate binding sites or other characteristics that might impact their use in experiments. More details..

DNA Analysis

Free Dna Sequence Analysis Software

MacVector provides a wide variety of useful DNA analysis tools, including base composition analysis, Restriction Enzyme searches, DNA Subsequence searches and 'Dot-Plot' comparisons between DNA:DNA and DNA:Protein sequences. A Coding Preference toolbox lets you select a variety of algorithms to graphically scan a DNA sequence for likely protein coding open reading frames. More details..

Protein Analysis

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Protein sequences can be reverse translated into DNA, compared using 'Dot-Plot' analysis and scanned for Proteolytic cleavage sites and amino acid sequence motifs. A comprehensive Protein Analysis Toolbox provides a wide variety of algorithms for analyzing the composition of proteins and presenting the results in graphical and tabular formats. More details..

Database Searching

MacVector has built-in Internet connectivity to the NCBI BLAST and Entrez databases. You can directly search Entrez for DNA or Protein sequences based on features, authors, keywords etc and directly download them into MacVector, complete with all features and annotations. The built-in BLAST interface lets you submit multiple BLAST jobs using DNA or Protein sequences and then download any matching sequences by selecting them from a hit list. Even without an Internet connection, MacVector can align sequences against any folder on your hard drive using a FastA algorithm, allowing for 'local' database searches. More details..

Multiple Sequence Alignment

You can align unlimited numbers of DNA or Protein sequences using the ClustalW algorithm built in to MacVector. A full-featured editor lets you make manual adjustments to the alignments and view them using a wide variety of customizable color schemes. You can create publication quality graphical outputs of the alignments and view pairwise combinations of the sequences in aligned and matrix formats. More details..

Sequence Assembly

Sequence Assembly functionality in MacVector is now provided by two modules. First there is a built-in function called Align To Reference. This can be used for two different functions, Sequence Confirmation and cDNA Alignment. This is included with MacVector and allows you to import trace files or sequence files and assemble them against a template sequence. This is ideal for small scale sequencing projects, especially resequencing. For example, checking an in situ mutagenesis experiment, a construct you've just made, or confirming the sequence of a cloned PCR fragment. It's also an excellent tool for SNP analysis, with some special tools to allow you to easily spot mutations from your original template sequence.

For full scale sequencing projects where you do not know the sequence you'll need MacVector and a separately purchased plugin called Assembler. This is a full contig assembly application that uses the phred, phrap and cross_match algorithms from the University of Washington to assembles traces into contigs. It displays full quality scores of the reads and the aligned contigs. The trial version of MacVector also includes the Assembler plugin. Click here to go to the Assembler product page.

Please note that AssemblyLIGN is no longer included with MacVector.

This is a list of computer software which is made for bioinformatics and released under open-source software licenses with articles in Wikipedia.

See also[edit]

References[edit]

1. ^Biopython License
2. ^'GeWorkbench License'. geWorkbench. Columbia University. 15 June 2014.

External links[edit]

• Free Biology Software – Free Software Directory – Free Software Foundation

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Dna Sequence Analysis Tools